A precise, simple, accurate and specific ratio derivative UV spectrophotometric method were developed and validated for routine quantitation of Tamsulosin Hydrochloride (TAM) and Finasteride (FINA) in combined dosage form. The first derivative ratio spectrophotometric method, zero order spectra of Tamsulosin Hydrochloride and Finasteride were quite parallel and overlapping. Hence first derivative ratio spectrophotometry method was possible for simultaneous estimation of these drugs. Also matrix effect can be reduced by this method. In first derivative ratio spectra, estimation was done at peak minima of Tamsulosin Hydrochloride and Finasteride at 235.92 nm and 253.94 nm respectively. The calibration graphs were linear in the concentration range of 1.6-8.0 μg/mL for Tamsulosin Hydrochloride and 10-60 μg/mL for Finasteride with mean recoveries of 98.05-100.62 % and 98.02-100.79 % for Tamsulosin Hydrochloride and Finasteride respectively.
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